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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
Fecha : |
21/02/2014 |
Actualizado : |
05/11/2020 |
Autor : |
Millot, J.C. ; Acosta, Y. ; Rebuffo, M. |
Título : |
Comparación de mezclas vs líneas puras de avena byzantina en producción de forraje, heno y grano |
Fecha de publicación : |
1980 |
Fuente / Imprenta : |
ln: Reunión Técnica, 3 : 1980 Dic 6-8 : Montevideo Montevideo (Uruguay): Facultad de Agronomía, 1980. |
Páginas : |
p84 |
Idioma : |
Español |
Thesagro : |
AVENA BYZANTINA; FACTORES DE PRODUCCION; FECHA DE SIEMBRA; GRANOS PIENSO; HENO; PRODUCCION DE PIENSOS; SIEMBRA. |
Asunto categoría : |
-- A50 Investigación agraria |
Marc : |
LEADER 00701naa a2200229 a 4500 001 1048477 005 2020-11-05 008 1980 bl uuuu u00u1 u #d 100 1 $aMILLOT, J.C. 245 $aComparación de mezclas vs líneas puras de avena byzantina en producción de forraje, heno y grano 260 $c1980 300 $ap84 650 $aAVENA BYZANTINA 650 $aFACTORES DE PRODUCCION 650 $aFECHA DE SIEMBRA 650 $aGRANOS PIENSO 650 $aHENO 650 $aPRODUCCION DE PIENSOS 650 $aSIEMBRA 700 1 $aACOSTA, Y. 700 1 $aREBUFFO, M. 773 $tln: Reunión Técnica, 3 : 1980 Dic 6-8 : Montevideo Montevideo (Uruguay): Facultad de Agronomía, 1980.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
01/10/2014 |
Actualizado : |
09/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
A - 1 |
Autor : |
ALQUEZAR, B.; RODRIGO, M.J.; LADO, J.; ZACARÍAS, L. |
Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.). |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
Tree Genetics & Genomes, 2013, v.9, no.5, p.1257-1269. |
ISSN : |
1614-2942 |
DOI : |
10.1007/s11295-013-0635-7 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013. |
Contenido : |
ABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit.
© 2013 Springer-Verlag Berlin Heidelberg. MenosABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high... Presentar Todo |
Palabras claves : |
ABA; CAROTENOIDS; GENE EXPRESSION; GRAPEFRUIT; LYCOPENE. |
Thesagro : |
CAROTENO; CITRUS. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02702naa a2200277 a 4500 001 1050762 005 2019-10-09 008 2013 bl uuuu u00u1 u #d 022 $a1614-2942 024 7 $a10.1007/s11295-013-0635-7$2DOI 100 1 $aALQUEZAR, B. 245 $aA comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.).$h[electronic resource] 260 $c2013 500 $aArticle history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013. 520 $aABSTRACT. Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit. © 2013 Springer-Verlag Berlin Heidelberg. 650 $aCAROTENO 650 $aCITRUS 653 $aABA 653 $aCAROTENOIDS 653 $aGENE EXPRESSION 653 $aGRAPEFRUIT 653 $aLYCOPENE 700 1 $aRODRIGO, M.J. 700 1 $aLADO, J. 700 1 $aZACARÍAS, L. 773 $tTree Genetics & Genomes, 2013$gv.9, no.5, p.1257-1269.
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